› 1gnq

X-ray diffraction
2.5Å resolution

X-RAY CRYSTAL STRUCTURE ANALYSIS OF THE CATALYTIC DOMAIN OF THE ONCOGENE PRODUCT P21H-RAS COMPLEXED WITH CAGED GTP AND MANT DGPPNHP

Released:
DOI: 10.2210/pdb1gnq/pdb
PDB entry 1gnq coloured by chain, front view.
PDB entry 1gnq coloured by chain, side view.
PDB entry 1gnq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
X-ray crystal structure analysis of the catalytic domain of the oncogene product p21H-ras complexed with caged GTP and mant dGppNHp.
Scheidig AJ, Franken SM, Corrie JE, Reid GP, Wittinghofer A, Pai EF, Goody RS
J Mol Biol 253 132-50 (1995)
PMID: 7473708

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133977 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase HRas, N-terminally processed Chain: A
Molecule details ›
Chain: A
Length: 166 amino acids
Theoretical weight: 18.88 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01112 (Residues: 1-166; Coverage: 88%)
Gene names: HRAS, HRAS1
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand CAG 1 x CAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P3221
Unit cell:
a: 40.8Å b: 40.8Å c: 158.2Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.207 0.207 0.269
Expression system: Not provided

Quick links

Citations

4 review citations

G protein mechanisms: insights from structural analysis.
Sprang SR. (1997)
3 more

2 mentions without citation

Ras conformational switching: simulating nucleotide-dependent conformational transitions with accelerated molecular dynamics.
Grant et al. (2009)
1 more