› 1gpi

X-ray diffraction
1.32Å resolution

Cellobiohydrolase Cel7D (CBH 58) from Phanerochaete chrysosporium. Catalytic module at 1.32 Angstrom resolution

Released:
DOI: 10.2210/pdb1gpi/pdb
PDB entry 1gpi coloured by chain, front view.
PDB entry 1gpi coloured by chain, side view.
PDB entry 1gpi coloured by chain, top view.
Source organism: Phanerodontia chrysosporium
Primary publication:
Family 7 cellobiohydrolases from Phanerochaete chrysosporium: crystal structure of the catalytic module of Cel7D (CBH58) at 1.32 A resolution and homology models of the isozymes.
Muñoz IG, Ubhayasekera W, Henriksson H, Szabó I, Pettersson G, Johansson G, Mowbray SL, Ståhlberg J
J Mol Biol 314 1097-111 (2001)
PMID: 11743726

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181655 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucanase Chain: A
Molecule details ›
Chain: A
Length: 431 amino acids
Theoretical weight: 45.78 KDa
Source organism: Phanerodontia chrysosporium
UniProt:
  • Canonical: Q7LIJ0 (Residues: 19-449; Coverage: 88%)
Gene names: CBH, CBHI.2, cbh1-4
Sequence domains: Glycosyl hydrolase family 7
Structure domains: Glycoside hydrolase, family 7, domain

Ligands and Environments

1 bound ligand:
Ligand NAG 1 x NAG
1 modified residue:
Ligand PCA 1 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: C2
Unit cell:
a: 86.307Å b: 46.625Å c: 99.079Å
α: 90° β: 103.19° γ: 90°
R-values:
R R work R free
0.216 0.213 0.242

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Citations

3 review citations

Degradation of cellulose by basidiomycetous fungi.
Baldrian et al. (2008)
2 more

12 mentions without citation

Plant-polysaccharide-degrading enzymes from Basidiomycetes.
Rytioja et al. (2014)
11 more