› 1gs0

X-ray diffraction
2.8Å resolution

Crystal structure of the catalytic fragment of murine poly(ADP-ribose) polymerase-2

Released:
DOI: 10.2210/pdb1gs0/pdb
PDB entry 1gs0 coloured by chain, front view.
PDB entry 1gs0 coloured by chain, side view.
PDB entry 1gs0 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal structure of the catalytic fragment of murine poly(ADP-ribose) polymerase-2.
Oliver AW, Amé JC, Roe SM, Good V, de Murcia G, Pearl LH
Nucleic Acids Res 32 456-64 (2004)
PMID: 14739238

Function and Biology Details

Reaction catalysed: 
NAD(+) + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131557 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Poly [ADP-ribose] polymerase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 351 amino acids
Theoretical weight: 39.7 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O88554 (Residues: 207-557; Coverage: 63%)
Gene names: Adprt2, Adprtl2, Aspartl2, Parp2
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 83.65Å b: 85.82Å c: 139.46Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.244 0.244 0.268
Expression system: Spodoptera frugiperda

Quick links

Citations

27 review citations

Poly(ADP-ribose): novel functions for an old molecule.
Schreiber et al. (2006)
26 more

6 mentions without citation

Molecular mechanism of poly(ADP-ribosyl)ation by PARP1 and identification of lysine residues as ADP-ribose acceptor sites.
Altmeyer et al. (2009)
5 more