› 1gsk

X-ray diffraction
1.7Å resolution

Crystal structure of CotA, an endospore coat protein from Bacillus subtilis

Released:
DOI: 10.2210/pdb1gsk/pdb
PDB entry 1gsk coloured by chain, front view.
PDB entry 1gsk coloured by chain, side view.
PDB entry 1gsk coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Crystal structure of a bacterial endospore coat component. A laccase with enhanced thermostability properties.
Enguita FJ, Martins LO, Henriques AO, Carrondo MA
J Biol Chem 278 19416-25 (2003)
PMID: 12637519

Function and Biology Details

Reactions catalysed: 
4 benzenediol + O(2) = 4 benzosemiquinone + 2 H(2)O
2 bilirubin + O(2) = 2 biliverdin + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139758 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Laccase Chain: A
Molecule details ›
Chain: A
Length: 513 amino acids
Theoretical weight: 58.57 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P07788 (Residues: 1-513; Coverage: 100%)
Gene names: BSU06300, cotA, pig
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

4 bound ligands:
Ligand GOL 4 x GOL
Ligand CU 1 x CU
Ligand C2O 1 x C2O
Ligand C1O 1 x C1O
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P3121
Unit cell:
a: 102.051Å b: 102.051Å c: 136.393Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.179 0.178 0.199
Expression system: Escherichia coli BL21

Quick links

Citations

25 review citations

Fungal laccases - occurrence and properties.
Baldrian P. (2006)
24 more

25 mentions without citation

Laccases: structure, function, and potential application in water bioremediation.
Arregui et al. (2019)
24 more