› 1h5a

X-ray diffraction
1.6Å resolution

STRUCTURE OF FERRIC HORSERADISH PEROXIDASE C1A IN COMPLEX WITH ACETATE

Released:
DOI: 10.2210/pdb1h5a/pdb
PDB entry 1h5a coloured by chain, front view.
PDB entry 1h5a coloured by chain, side view.
PDB entry 1h5a coloured by chain, top view.
Source organism: Armoracia rusticana
Primary publication:
The catalytic pathway of horseradish peroxidase at high resolution.
Berglund GI, Carlsson GH, Smith AT, Szöke H, Henriksen A, Hajdu J
Nature 417 463-8 (2002)
PMID: 12024218

Function and Biology Details

Reaction catalysed: 
2 phenolic donor + H(2)O(2) = 2 phenoxyl radical of the donor + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132478 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peroxidase C1A Chain: A
Molecule details ›
Chain: A
Length: 308 amino acids
Theoretical weight: 33.95 KDa
Source organism: Armoracia rusticana
Expression system: Escherichia coli
UniProt:
  • Canonical: P00433 (Residues: 31-338; Coverage: 95%)
Gene names: HPRC1, PRXC1A
Sequence domains: Peroxidase
Structure domains:

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
2 bound ligands:
Ligand CA 2 x CA
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P212121
Unit cell:
a: 40.347Å b: 67.774Å c: 117.265Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.178 0.202
Expression system: Escherichia coli

Quick links

Citations

45 review citations

Heme enzyme structure and function.
Poulos TL. (2014)
44 more

15 mentions without citation

An updated view on horseradish peroxidases: recombinant production and biotechnological applications.
Krainer et al. (2015)
14 more