› 1h65

X-ray diffraction
2Å resolution

Crystal structure of pea Toc34 - a novel GTPase of the chloroplast protein translocon

Released:
DOI: 10.2210/pdb1h65/pdb
PDB entry 1h65 coloured by chain, front view.
PDB entry 1h65 coloured by chain, side view.
PDB entry 1h65 coloured by chain, top view.
Source organism: Pisum sativum
Primary publication:
Crystal structure of pea Toc34, a novel GTPase of the chloroplast protein translocon.
Sun YJ, Forouhar F, Li Hm HM, Tu SL, Yeh YH, Kao S, Shr HL, Chou CC, Chen C, Hsiao CD
Nat Struct Biol 9 95-100 (2002)
PMID: 11753431

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174941 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Translocase of chloroplast 34 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 270 amino acids
Theoretical weight: 29.77 KDa
Source organism: Pisum sativum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q41009 (Residues: 1-258; Coverage: 83%)
Gene names: IAP34, OEP34, TOC34
Sequence domains: AIG1 family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand GDP 3 x GDP
Ligand MG 3 x MG
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-18B
Spacegroup: C2
Unit cell:
a: 143.16Å b: 78.673Å c: 67.279Å
α: 90° β: 91.44° γ: 90°
R-values:
R R work R free
0.186 0.186 0.23
Expression system: Escherichia coli

Quick links

Citations

36 review citations

Protein import into chloroplasts.
Soll et al. (2004)
35 more

3 mentions without citation

Structural basis of oligomerization in septin-like GTPase of immunity-associated protein 2 (GIMAP2).
Schwefel et al. (2010)
2 more