› 1htz

X-ray diffraction
2.4Å resolution

CRYSTAL STRUCTURE OF TEM52 BETA-LACTAMASE

Released:
DOI: 10.2210/pdb1htz/pdb
PDB entry 1htz coloured by chain, front view.
PDB entry 1htz coloured by chain, side view.
PDB entry 1htz coloured by chain, top view.
Source organism: Klebsiella pneumoniae
Primary publication:
Predicting the emergence of antibiotic resistance by directed evolution and structural analysis.
Orencia MC, Yoon JS, Ness JE, Stemmer WP, Stevens RC
Nat Struct Biol 8 238-42 (2001)
PMID: 11224569

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193108 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 263 amino acids
Theoretical weight: 28.94 KDa
Source organism: Klebsiella pneumoniae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9R435 (Residues: 24-286; Coverage: 100%)
Gene name: blaTEM-52
Sequence domains:
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P43212
Unit cell:
a: 88.382Å b: 88.382Å c: 500.395Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.217 0.261
Expression system: Escherichia coli BL21

Quick links

Citations

26 review citations

Three decades of beta-lactamase inhibitors.
Drawz et al. (2010)
25 more

10 mentions without citation

Structural and Mechanistic Basis for Extended-Spectrum Drug-Resistance Mutations in Altering the Specificity of TEM, CTX-M, and KPC β-lactamases.
Palzkill T. (2018)
9 more