› 1hwh

X-ray diffraction
2.9Å resolution

1:1 COMPLEX OF HUMAN GROWTH HORMONE MUTANT G120R WITH ITS SOLUBLE BINDING PROTEIN

Released:
DOI: 10.2210/pdb1hwh/pdb
PDB entry 1hwh coloured by chain, front view.
PDB entry 1hwh coloured by chain, side view.
PDB entry 1hwh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of an antagonist mutant of human growth hormone, G120R, in complex with its receptor at 2.9 A resolution.
Sundström M, Lundqvist T, Rödin J, Giebel LB, Milligan D, Norstedt G
J Biol Chem 271 32197-203 (1996)
PMID: 8943276

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134132 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Somatotropin Chain: A
Molecule details ›
Chain: A
Length: 191 amino acids
Theoretical weight: 22.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01241 (Residues: 27-217; Coverage: 100%)
Gene name: GH1
Sequence domains: Somatotropin hormone family
Structure domains: Growth Hormone; Chain: A;
Growth hormone-binding protein Chain: B
Molecule details ›
Chain: B
Length: 237 amino acids
Theoretical weight: 27.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P10912 (Residues: 19-255; Coverage: 38%)
Gene name: GHR
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P43212
Unit cell:
a: 67.78Å b: 67.78Å c: 229.24Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.215 0.317
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Interleukin-6: structure-function relationships.
Simpson et al. (1997)
3 more

5 mentions without citation

Crystal structure of an affinity-matured prolactin complexed to its dimerized receptor reveals the topology of hormone binding site 2.
Broutin et al. (2010)
4 more