› 1hze

Solution NMR

SOLUTION STRUCTURE OF THE N-TERMINAL DOMAIN OF RIBOFLAVIN SYNTHASE FROM E. COLI

Released:
DOI: 10.2210/pdb1hze/pdb
PDB entry 1hze coloured by chain, front view.
PDB entry 1hze coloured by chain, side view.
PDB entry 1hze coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The solution structure of the N-terminal domain of riboflavin synthase.
Truffault V, Coles M, Diercks T, Abelmann K, Eberhardt S, Lüttgen H, Bacher A, Kessler H
J Mol Biol 309 949-60 (2001)
PMID: 11399071

Function and Biology Details

Reaction catalysed: 
2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142751 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Riboflavin synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 97 amino acids
Theoretical weight: 10.58 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AFU8 (Residues: 1-97; Coverage: 46%)
Gene names: JW1654, b1662, ribC, ribE
Sequence domains: Lumazine binding domain
Structure domains: Elongation Factor Tu (Ef-tu); domain 3

Ligands and Environments

1 bound ligand:
Ligand RBF 2 x RBF
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Escherichia coli

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Citations

6 review citations

Biosynthesis of flavocoenzymes.
Fischer et al. (2005)
5 more

1 mention without citation

Enhancer viruses for combinatorial cell-subclass-specific labeling.
Graybuck et al. (2021)