› 1i8b

X-ray diffraction
1.95Å resolution

Chalcone synthase (G256F)

Released:
DOI: 10.2210/pdb1i8b/pdb
PDB entry 1i8b coloured by chain, front view.
PDB entry 1i8b coloured by chain, side view.
PDB entry 1i8b coloured by chain, top view.
Source organism: Medicago sativa
Primary publication:
Structure-guided programming of polyketide chain-length determination in chalcone synthase.
Jez JM, Bowman ME, Noel JP
Biochemistry 40 14829-38 (2001)
PMID: 11732902

Function and Biology Details

Reaction catalysed: 
3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151628 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chalcone synthase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 389 amino acids
Theoretical weight: 42.88 KDa
Source organism: Medicago sativa
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P30074 (Residues: 1-389; Coverage: 100%)
Gene name: CHS2
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CSD 2 x CSD

Experiments and Validation Details

Entry percentile scores
X-ray source: MACSCIENCE
Spacegroup: P3121
Unit cell:
a: 97.964Å b: 97.964Å c: 130.305Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.201 0.201 0.251
Expression system: Escherichia coli BL21

Quick links

Citations

12 review citations

Structure and function of the chalcone synthase superfamily of plant type III polyketide synthases.
Abe et al. (2010)
11 more