› 1iog

Solution NMR

INSULIN MUTANT A3 GLY,(B1, B10, B16, B27)GLU, DES-B30, NMR, 19 STRUCTURES

Released:
DOI: 10.2210/pdb1iog/pdb
PDB entry 1iog coloured by chain, ensemble of 19 models, front view.
PDB entry 1iog coloured by chain, ensemble of 19 models, side view.
PDB entry 1iog coloured by chain, ensemble of 19 models, top view.
Source organism: Homo sapiens
Primary publication:
The relationship between insulin bioactivity and structure in the NH2-terminal A-chain helix.
Olsen HB, Ludvigsen S, Kaarsholm NC
J Mol Biol 284 477-88 (1998)
PMID: 9813131

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134182 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chain: A
Molecule details ›
Chain: A
Length: 21 amino acids
Theoretical weight: 2.34 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P01308 (Residues: 90-110; Coverage: 24%)
Gene name: INS
Insulin B chain Chain: B
Molecule details ›
Chain: B
Length: 29 amino acids
Theoretical weight: 3.3 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P01308 (Residues: 25-53; Coverage: 34%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: distance geometry
Expression system: Saccharomyces cerevisiae

Quick links

Citations

2 review citations

Expression of insulin in yeast: the importance of molecular adaptation for secretion and conversion.
Kjeldsen et al. (2001)
1 more

1 mention without citation

High precision protein functional site detection using 3D convolutional neural networks.
Torng et al. (2019)