› 1ioz

X-ray diffraction
2Å resolution

Crystal Structure of the C-HA-RAS Protein Prepared by the Cell-Free Synthesis

Released:
DOI: 10.2210/pdb1ioz/pdb
PDB entry 1ioz coloured by chain, front view.
PDB entry 1ioz coloured by chain, side view.
PDB entry 1ioz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Selenomethionine incorporation into a protein by cell-free synthesis.
Kigawa T, Yamaguchi-Nunokawa E, Kodama K, Matsuda T, Yabuki T, Matsuda N, Ishitani R, Nureki O, Yokoyama S
J Struct Funct Genomics 2 29-35 (2002)
PMID: 12836672

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133956 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase HRas Chain: A
Molecule details ›
Chain: A
Length: 171 amino acids
Theoretical weight: 19.52 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01112 (Residues: 1-171; Coverage: 91%)
Gene names: HRAS, HRAS1
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand GDP 1 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: P6522
Unit cell:
a: 82.645Å b: 82.645Å c: 102.794Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.285 0.238 0.283
Expression system: Not provided

Quick links

Citations

14 review citations

Protein expression systems for structural genomics and proteomics.
Yokoyama S. (2003)
13 more

6 mentions without citation

Peroxisomal lactate dehydrogenase is generated by translational readthrough in mammals.
Schueren et al. (2014)
5 more