› 1iuu

X-ray diffraction
2Å resolution

P-HYDROXYBENZOATE HYDROXYLASE COMPLEXED WITH 4-AMINOBENZOATE AT PH 9.4

Released:
DOI: 10.2210/pdb1iuu/pdb
PDB entry 1iuu coloured by chain, front view.
PDB entry 1iuu coloured by chain, side view.
PDB entry 1iuu coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
pH-dependent structural changes in the active site of p-hydroxybenzoate hydroxylase point to the importance of proton and water movements during catalysis.
Gatti DL, Entsch B, Ballou DP, Ludwig ML
Biochemistry 35 567-78 (1996)
PMID: 8555229

Function and Biology Details

Reaction catalysed: 
4-hydroxybenzoate + NADPH + O(2) = protocatechuate + NADP(+) + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149007 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
p-hydroxybenzoate hydroxylase Chain: A
Molecule details ›
Chain: A
Length: 394 amino acids
Theoretical weight: 44.38 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Not provided
UniProt:
  • Canonical: P20586 (Residues: 1-394; Coverage: 100%)
Gene names: PA0247, pobA
Sequence domains: FAD binding domain
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
1 bound ligand:
Ligand PAB 1 x PAB
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2221
Unit cell:
a: 71.89Å b: 146.54Å c: 87.94Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.176 not available
Expression system: Not provided

Quick links

Citations

2 review citations

Protein dynamics and electrostatics in the function of p-hydroxybenzoate hydroxylase.
Entsch et al. (2005)
1 more

1 mention without citation

Expression, purification, crystallization and initial crystallographic characterization of the p-hydroxybenzoate hydroxylase from Corynebacterium glutamicum.
Kwon et al. (2007)