› 1j1f

X-ray diffraction
1.6Å resolution

Crystal structure of the RNase MC1 mutant N71T in complex with 5'-GMP

Released:
DOI: 10.2210/pdb1j1f/pdb
PDB entry 1j1f coloured by chain, front view.
PDB entry 1j1f coloured by chain, side view.
PDB entry 1j1f coloured by chain, top view.
Source organism: Momordica charantia
Primary publication:
Crystal structures of the ribonuclease MC1 mutants N71T and N71S in complex with 5'-GMP: structural basis for alterations in substrate specificity.
Numata T, Suzuki A, Kakuta Y, Kimura K, Yao M, Tanaka I, Yoshida Y, Ueda T, Kimura M
Biochemistry 42 5270-8 (2003)
PMID: 12731868

Function and Biology Details

Reaction catalysed: 
(1a) RNA = an (RNA fragment)-3'-nucleoside-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA fragment)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150006 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease MC Chain: A
Molecule details ›
Chain: A
Length: 191 amino acids
Theoretical weight: 21.34 KDa
Source organism: Momordica charantia
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P23540 (Residues: 1-191; Coverage: 100%)
Gene name: MC1
Sequence domains: Ribonuclease T2 family
Structure domains: Ribonuclease T2-like

Ligands and Environments

1 bound ligand:
Ligand 5GP 1 x 5GP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: P212121
Unit cell:
a: 38.375Å b: 65.777Å c: 75.882Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.221 0.244
Expression system: Komagataella pastoris

Quick links

Citations

1 review citation

T2 Family ribonucleases: ancient enzymes with diverse roles.
Luhtala et al. (2010)
9 other articles

1 mention without citation

Variation among S-locus haplotypes and among stylar RNases in almond.
Goonetilleke et al. (2020)