› 1j3r

X-ray diffraction
2.18Å resolution

Crystal structure of Thermococcus litoralis phosphogrucose isomerase complexed with gluconate-6-phosphate

Released:
DOI: 10.2210/pdb1j3r/pdb
PDB entry 1j3r coloured by chain, front view.
PDB entry 1j3r coloured by chain, side view.
PDB entry 1j3r coloured by chain, top view.
Source organism: Thermococcus litoralis
Entry authors: Jeong J-J, Fushinobu S, Ito S, Hidaka M, Shoun H, Wakagi T

Function and Biology Details

Reaction catalysed: 
Alpha-D-glucose 6-phosphate = beta-D-fructofuranose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160839 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucose-6-phosphate isomerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 190 amino acids
Theoretical weight: 21.75 KDa
Source organism: Thermococcus litoralis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P84140 (Residues: 1-190; Coverage: 100%)
Gene name: pgiA
Sequence domains: Glucose-6-phosphate isomerase (GPI)
Structure domains: Jelly Rolls

Ligands and Environments

2 bound ligands:
Ligand 6PG 2 x 6PG
Ligand FE 1 x FE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU ULTRAX 18
Spacegroup: P212121
Unit cell:
a: 40.67Å b: 80.23Å c: 130.04Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.218 0.263
Expression system: Escherichia coli BL21(DE3)

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