› 1ji1

X-ray diffraction
1.6Å resolution

Crystal Structure Analysis of Thermoactinomyces vulgaris R-47 alpha-Amylase 1

Released:
DOI: 10.2210/pdb1ji1/pdb
PDB entry 1ji1 coloured by chain, front view.
PDB entry 1ji1 coloured by chain, side view.
PDB entry 1ji1 coloured by chain, top view.
Source organism: Thermoactinomyces vulgaris
Primary publication:
Crystal structures and structural comparison of Thermoactinomyces vulgaris R-47 alpha-amylase 1 (TVAI) at 1.6 A resolution and alpha-amylase 2 (TVAII) at 2.3 A resolution.
Kamitori S, Abe A, Ohtaki A, Kaji A, Tonozuka T, Sakano Y
J Mol Biol 318 443-53 (2002)
PMID: 12051850

Function and Biology Details

Reaction catalysed: 
Hydrolysis of pullulan to panose (6-alpha-D-glucosylmaltose)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178752 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neopullulanase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 637 amino acids
Theoretical weight: 71.11 KDa
Source organism: Thermoactinomyces vulgaris
Expression system: Escherichia coli
UniProt:
  • Canonical: Q60053 (Residues: 30-666; Coverage: 100%)
Gene name: tvaI
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CA 6 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P21
Unit cell:
a: 122.57Å b: 50.84Å c: 107.8Å
α: 90° β: 104.2° γ: 90°
R-values:
R R work R free
0.182 0.182 0.206
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Pullulan degrading enzymes of bacterial origin.
Domań-Pytka et al. (2004)
3 more

5 mentions without citation

α-N-acetylgalactosaminidase from infant-associated bifidobacteria belonging to novel glycoside hydrolase family 129 is implicated in alternative mucin degradation pathway.
Kiyohara et al. (2012)
4 more