› 1jiz

X-ray diffraction
2.6Å resolution

Crystal Structure Analysis of human Macrophage Elastase MMP-12

Released:
DOI: 10.2210/pdb1jiz/pdb
PDB entry 1jiz coloured by chain, front view.
PDB entry 1jiz coloured by chain, side view.
PDB entry 1jiz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human macrophage elastase (MMP-12) in complex with a hydroxamic acid inhibitor.
Nar H, Werle K, Bauer MM, Dollinger H, Jung B
J Mol Biol 312 743-51 (2001)
PMID: 11575929

Function and Biology Details

Reaction catalysed: 
Hydrolysis of soluble and insoluble elastin (1). Specific cleavages are also produced at -Ala(14)-|-Leu- and -Tyr(16)-|-Leu- in the B chain of insulin (2).
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153971 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Macrophage metalloelastase Chains: A, B
Molecule details ›
Chains: A, B
Length: 166 amino acids
Theoretical weight: 18.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P39900 (Residues: 100-264; Coverage: 36%)
Gene names: HME, MMP12
Sequence domains: Matrixin
Structure domains: Collagenase (Catalytic Domain)

Ligands and Environments

3 bound ligands:
Ligand ZN 4 x ZN
Ligand CA 6 x CA
Ligand CGS 2 x CGS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: I222
Unit cell:
a: 67.4Å b: 87.2Å c: 169.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.197 0.256
Expression system: Escherichia coli

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Citations

3 review citations

Structural basis of the matrix metalloproteinases and their physiological inhibitors, the tissue inhibitors of metalloproteinases.
Bode et al. (2003)
2 more

10 mentions without citation

Structure of trans-resveratrol in complex with the cardiac regulatory protein troponin C.
Pineda-Sanabria et al. (2011)
9 more