› 1jl2

X-ray diffraction
1.76Å resolution

Crystal structure of TCEO RNase H-a chimera combining the folding core from T. thermophilus RNase H and the remaining region of E. coli RNase H

Released:
DOI: 10.2210/pdb1jl2/pdb
PDB entry 1jl2 coloured by chain, front view.
PDB entry 1jl2 coloured by chain, side view.
PDB entry 1jl2 coloured by chain, top view.
Source organisms:
Primary publication:
Contributions of folding cores to the thermostabilities of two ribonucleases H.
Robic S, Berger JM, Marqusee S
Protein Sci 11 381-9 (2002)
PMID: 11790848

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141633 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease H; Ribonuclease HI Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 156 amino acids
Theoretical weight: 17.6 KDa
Source organisms: Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0A7Y4 (Residues: 1-49, 125-155; Coverage: 52%)
  • Canonical: P29253 (Residues: 54-129; Coverage: 46%)
Gene names: JW0204, TTHA1556, b0214, dasF, herA, rnh, rnhA, sdrA
Sequence domains: RNase H
Structure domains: Ribonuclease H-like superfamily/Ribonuclease H

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21
Unit cell:
a: 56.195Å b: 87.033Å c: 60.687Å
α: 90° β: 91.87° γ: 90°
R-values:
R R work R free
0.249 0.249 0.286
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

Heat capacity in proteins.
Prabhu et al. (2005)
2 more

3 mentions without citation

Snake Venom Metalloproteinases (SVMPs): A structure-function update.
Olaoba et al. (2020)
2 more