› 1jr1

X-ray diffraction
2.6Å resolution

Crystal structure of Inosine Monophosphate Dehydrogenase in complex with Mycophenolic Acid

Released:
DOI: 10.2210/pdb1jr1/pdb
PDB entry 1jr1 coloured by chain, front view.
PDB entry 1jr1 coloured by chain, side view.
PDB entry 1jr1 coloured by chain, top view.
Source organism: Cricetulus griseus
Primary publication:
Structure and mechanism of inosine monophosphate dehydrogenase in complex with the immunosuppressant mycophenolic acid.
Sintchak MD, Fleming MA, Futer O, Raybuck SA, Chambers SP, Caron PR, Murcko MA, Wilson KP
Cell 85 921-30 (1996)
PMID: 8681386

Function and Biology Details

Reaction catalysed: 
Inosine 5'-phosphate + NAD(+) + H(2)O = xanthosine 5'-phosphate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146111 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inosine-5'-monophosphate dehydrogenase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 514 amino acids
Theoretical weight: 55.96 KDa
Source organism: Cricetulus griseus
Expression system: Escherichia coli
UniProt:
  • Canonical: P12269 (Residues: 1-514; Coverage: 100%)
Gene name: IMPDH2
Sequence domains:
Structure domains: Aldolase class I

Ligands and Environments

3 bound ligands:
Ligand K 2 x K
Ligand IMP 2 x IMP
Ligand MOA 2 x MOA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P4
Unit cell:
a: 110.6Å b: 110.6Å c: 111Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.217 0.285
Expression system: Escherichia coli

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Citations

47 review citations

The AMP-activated/SNF1 protein kinase subfamily: metabolic sensors of the eukaryotic cell?
Hardie et al. (1998)
46 more

16 mentions without citation

IMP dehydrogenase: structure, mechanism, and inhibition.
Hedstrom L. (2009)
15 more