› 1js1

X-ray diffraction
2Å resolution

Crystal Structure of a new transcarbamylase from the anaerobic bacterium Bacteroides fragilis at 2.0 A resolution

Released:
DOI: 10.2210/pdb1js1/pdb
PDB entry 1js1 coloured by chain, front view.
PDB entry 1js1 coloured by chain, side view.
PDB entry 1js1 coloured by chain, top view.
Source organism: Bacteroides fragilis
Primary publication:
Crystal structure of a transcarbamylase-like protein from the anaerobic bacterium Bacteroides fragilis at 2.0 A resolution.
Shi D, Gallegos R, DePonte J, Morizono H, Yu X, Allewell NM, Malamy M, Tuchman M
J Mol Biol 320 899-908 (2002)
PMID: 12095263

Function and Biology Details

Reaction catalysed: 
Carbamoyl phosphate + N(2)-succinyl-L-ornithine = phosphate + N-succinyl-L-citrulline
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183612 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-succinylornithine carbamoyltransferase Chains: X, Y, Z
Molecule details ›
Chains: X, Y, Z
Length: 324 amino acids
Theoretical weight: 37.28 KDa
Source organism: Bacteroides fragilis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8A1E9 (Residues: 1-317; Coverage: 100%)
Gene names: BT_3717, argF'
Sequence domains:
Structure domains: Aspartate/ornithine carbamoyltransferase

Ligands and Environments

1 bound ligand:
Ligand PO4 3 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P43212
Unit cell:
a: 153.411Å b: 153.411Å c: 94.842Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.206 0.252
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Surprising arginine biosynthesis: a reappraisal of the enzymology and evolution of the pathway in microorganisms.
Xu et al. (2007)
4 more

3 mentions without citation

Intricate knots in proteins: Function and evolution.
Virnau et al. (2006)
2 more