› 1k7e

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURE OF WILD-TYPE TRYPTOPHAN SYNTHASE COMPLEXED WITH N-[1H-INDOL-3-YL-ACETYL]GLYCINE ACID

Released:
DOI: 10.2210/pdb1k7e/pdb
PDB entry 1k7e coloured by chain, front view.
PDB entry 1k7e coloured by chain, side view.
PDB entry 1k7e coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Primary publication:
Crystal structures of a new class of allosteric effectors complexed to tryptophan synthase.
Weyand M, Schlichting I, Marabotti A, Mozzarelli A
J Biol Chem 277 10647-52 (2002)
PMID: 11756456

Function and Biology Details

Reaction catalysed: 
(1a) 1-C-(indol-3-yl)glycerol 3-phosphate = indole + D-glyceraldehyde 3-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133696 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tryptophan synthase alpha chain Chain: A
Molecule details ›
Chain: A
Length: 268 amino acids
Theoretical weight: 28.7 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Escherichia coli
UniProt:
  • Canonical: P00929 (Residues: 1-268; Coverage: 100%)
Gene names: STM1727, trpA
Sequence domains: Tryptophan synthase alpha chain
Structure domains: Aldolase class I
Tryptophan synthase beta chain Chain: B
Molecule details ›
Chain: B
Length: 396 amino acids
Theoretical weight: 42.79 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A2K1 (Residues: 2-397; Coverage: 100%)
Gene names: STM1726, trpB
Sequence domains: Pyridoxal-phosphate dependent enzyme
Structure domains: Rossmann fold

Ligands and Environments


Cofactor: Ligand PLP 1 x PLP
2 bound ligands:
Ligand IAG 1 x IAG
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: C2
Unit cell:
a: 182.651Å b: 59.081Å c: 67.3Å
α: 90° β: 94.55° γ: 90°
R-values:
R R work R free
0.171 0.167 0.244
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Tryptophan synthase: the workings of a channeling nanomachine.
Dunn et al. (2008)
1 more

2 mentions without citation

Preference of small molecules for local minimum conformations when binding to proteins.
Wang et al. (2007)
1 more