› 1k8d

X-ray diffraction
2.3Å resolution

crystal structure of the non-classical MHC class Ib Qa-2 complexed with a self peptide

Released:
DOI: 10.2210/pdb1k8d/pdb
PDB entry 1k8d coloured by chain, front view.
PDB entry 1k8d coloured by chain, side view.
PDB entry 1k8d coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Promiscuous antigen presentation by the nonclassical MHC Ib Qa-2 is enabled by a shallow, hydrophobic groove and self-stabilized peptide conformation.
He X, Tabaczewski P, Ho J, Stroynowski I, Garcia KC
Structure 9 1213-24 (2001)
PMID: 11738047

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-135106 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
H-2 class I histocompatibility antigen, Q7 alpha chain Chain: A
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 31.63 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P14429 (Residues: 22-295; Coverage: 88%)
Gene name: H2-Q7
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 99 amino acids
Theoretical weight: 11.7 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01887 (Residues: 21-119; Coverage: 100%)
Gene name: B2m
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Large ribosomal subunit protein eL19 Chain: P
Molecule details ›
Chain: P
Length: 9 amino acids
Theoretical weight: 1.14 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: P84099 (Residues: 137-145; Coverage: 5%)
Gene name: Rpl19

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 53.92Å b: 56.18Å c: 119.92Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.222 0.278
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

9 review citations

MHC class Ib molecules bridge innate and acquired immunity.
Rodgers et al. (2005)
8 more

4 mentions without citation

Roles for major histocompatibility complex glycosylation in immune function.
Ryan et al. (2012)
3 more