› 1kfi

X-ray diffraction
2.4Å resolution

Crystal Structure of the Exocytosis-Sensitive Phosphoprotein, pp63/Parafusin (phosphoglucomutase) from Paramecium

Released:
DOI: 10.2210/pdb1kfi/pdb
PDB entry 1kfi coloured by chain, front view.
PDB entry 1kfi coloured by chain, side view.
PDB entry 1kfi coloured by chain, top view.
Source organism: Paramecium tetraurelia
Primary publication:
Crystal structure analysis of the exocytosis-sensitive phosphoprotein, pp63/parafusin (phosphoglucomutase), from Paramecium reveals significant conformational variability.
Müller S, Diederichs K, Breed J, Kissmehl R, Hauser K, Plattner H, Welte W
J Mol Biol 315 141-53 (2002)
PMID: 11779235

Function and Biology Details

Reaction catalysed: 
Alpha-D-glucose 1-phosphate = alpha-D-glucose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155620 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P212121
Unit cell:
a: 67.346Å b: 133.609Å c: 150.545Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.227 0.286
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Mutations in hereditary phosphoglucomutase 1 deficiency map to key regions of enzyme structure and function.
Beamer LJ. (2015)
2 more

8 mentions without citation

Evolutionary trace analysis of the alpha-D-phosphohexomutase superfamily.
Shackelford et al. (2004)
7 more