› 1ku1

X-ray diffraction
1.93Å resolution

Crystal Structure of the Sec7 Domain of Yeast GEA2

Released:
DOI: 10.2210/pdb1ku1/pdb
PDB entry 1ku1 coloured by chain, front view.
PDB entry 1ku1 coloured by chain, side view.
PDB entry 1ku1 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Mechanism of domain closure of Sec7 domains and role in BFA sensitivity.
Renault L, Christova P, Guibert B, Pasqualato S, Cherfils J
Biochemistry 41 3605-12 (2002)
PMID: 11888276

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153994 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ARF guanine-nucleotide exchange factor 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 230 amino acids
Theoretical weight: 26.55 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P39993 (Residues: 558-766; Coverage: 14%)
Gene names: GEA2, YEL022W
Sequence domains: Sec7 domain
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LURE BEAMLINE DW32, MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: C2
Unit cell:
a: 125.052Å b: 75.881Å c: 76.199Å
α: 90° β: 105.37° γ: 90°
R-values:
R R work R free
0.22 0.214 0.228
Expression system: Escherichia coli

Quick links

Citations

2 review citations

IQSEC2 mutation update and review of the female-specific phenotype spectrum including intellectual disability and epilepsy.
Shoubridge et al. (2019)
1 more

2 mentions without citation

Regulating the large Sec7 ARF guanine nucleotide exchange factors: the when, where and how of activation.
Wright et al. (2014)
1 more