› 1kxe

X-ray diffraction
3.2Å resolution

SINDBIS VIRUS CAPSID (Y180S, E183G DOUBLE MUTANT), TETRAGONAL CRYSTAL FORM

Released:
DOI: 10.2210/pdb1kxe/pdb
PDB entry 1kxe coloured by chain, front view.
PDB entry 1kxe coloured by chain, side view.
PDB entry 1kxe coloured by chain, top view.
Source organism: Sindbis virus
Primary publication:
Structural analysis of Sindbis virus capsid mutants involving assembly and catalysis.
Choi HK, Lee S, Zhang YP, McKinney BR, Wengler G, Rossmann MG, Kuhn RJ
J Mol Biol 262 151-67 (1996)
PMID: 8831786

Function and Biology Details

Reaction catalysed: 
Autocatalytic release of the core protein from the N-terminus of the togavirus structural polyprotein by hydrolysis of a -Trp-|-Ser- bond.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136788 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Capsid protein Chain: A
Molecule details ›
Chain: A
Length: 159 amino acids
Theoretical weight: 17.27 KDa
Source organism: Sindbis virus
Expression system: Escherichia coli
UniProt:
  • Canonical: P03316 (Residues: 106-264; Coverage: 13%)
Sequence domains: Alphavirus core protein
Structure domains: Trypsin-like serine proteases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P43212
Unit cell:
a: 57Å b: 57Å c: 109.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.188 not available
Expression system: Escherichia coli

Quick links

Citations

4 review citations

A structural perspective of the flavivirus life cycle.
Mukhopadhyay et al. (2005)
3 more