› 1l9b

X-ray diffraction
2.4Å resolution

X-Ray Structure of the Cytochrome-c(2)-Photosynthetic Reaction Center Electron Transfer Complex from Rhodobacter sphaeroides in Type II Co-Crystals

Released:
DOI: 10.2210/pdb1l9b/pdb
PDB entry 1l9b coloured by chain, front view.
PDB entry 1l9b coloured by chain, side view.
PDB entry 1l9b coloured by chain, top view.
Source organism: Cereibacter sphaeroides
Primary publication:
X-ray structure determination of the cytochrome c2: reaction center electron transfer complex from Rhodobacter sphaeroides.
Axelrod HL, Abresch EC, Okamura MY, Yeh AP, Rees DC, Feher G
J Mol Biol 319 501-15 (2002)
PMID: 12051924

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-142930 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Reaction center protein L chain Chain: L
Molecule details ›
Chain: L
Length: 281 amino acids
Theoretical weight: 31.35 KDa
Source organism: Cereibacter sphaeroides
UniProt:
  • Canonical: P0C0Y8 (Residues: 2-282; Coverage: 100%)
Gene name: pufL
Sequence domains: Photosynthetic reaction centre protein
Structure domains: Photosystem II protein D1-like
Reaction center protein M chain Chain: M
Molecule details ›
Chain: M
Length: 307 amino acids
Theoretical weight: 34.4 KDa
Source organism: Cereibacter sphaeroides
UniProt:
  • Canonical: P0C0Y9 (Residues: 2-308; Coverage: 100%)
Gene name: pufM
Sequence domains: Photosynthetic reaction centre protein
Structure domains: Photosystem II protein D1-like
Reaction center protein H chain Chain: H
Molecule details ›
Chain: H
Length: 260 amino acids
Theoretical weight: 28.07 KDa
Source organism: Cereibacter sphaeroides
UniProt:
  • Canonical: P0C0Y7 (Residues: 1-260; Coverage: 100%)
Gene name: puhA
Sequence domains:
Structure domains:
Cytochrome c2 Chain: C
Molecule details ›
Chain: C
Length: 124 amino acids
Theoretical weight: 13.49 KDa
Source organism: Cereibacter sphaeroides
UniProt:
  • Canonical: P0C0X8 (Residues: 1-124; Coverage: 100%)
Gene name: cycA
Structure domains: Cytochrome c-like domain

Ligands and Environments

9 bound ligands:
Ligand NA 1 x NA
Ligand BCL 4 x BCL
Ligand BPH 2 x BPH
Ligand LDA 9 x LDA
Ligand HTO 2 x HTO
Ligand FE2 1 x FE2
Ligand CL 1 x CL
Ligand U10 1 x U10
Ligand HEM 1 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21
Unit cell:
a: 78.222Å b: 115.65Å c: 79.677Å
α: 90° β: 110.29° γ: 90°
R-values:
R R work R free
0.22 0.22 0.264

Quick links

Citations

14 review citations

Structure and function of photosystem I: interaction with its soluble electron carriers and external antenna systems.
Fromme et al. (2003)
13 more

20 mentions without citation

Catalysis and Electron Transfer in De Novo Designed Metalloproteins.
Koebke et al. (2022)
19 more