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X-ray diffraction
2.5Å resolution

STRUCTURE OF A TERNARY COMPLEX OF AN ALLOSTERIC LACTATE DEHYDROGENASE FROM BACILLUS STEAROTHERMOPHILUS AT 2.5 ANGSTROMS RESOLUTION

Released:
DOI: 10.2210/pdb1ldn/pdb
PDB entry 1ldn coloured by chain, front view.
PDB entry 1ldn coloured by chain, side view.
PDB entry 1ldn coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Structure of a ternary complex of an allosteric lactate dehydrogenase from Bacillus stearothermophilus at 2.5 A resolution.
Wigley DB, Gamblin SJ, Turkenburg JP, Dodson EJ, Piontek K, Muirhead H, Holbrook JJ
J Mol Biol 223 317-35 (1992)
PMID: 1731077

Function and Biology Details

Reaction catalysed: 
(S)-lactate + NAD(+) = pyruvate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132419 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-lactate dehydrogenase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 316 amino acids
Theoretical weight: 34.75 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Not provided
UniProt:
  • Canonical: P00344 (Residues: 1-316; Coverage: 100%)
Gene names: lct, ldh
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 8 x NAD
2 bound ligands:
Ligand FBP 4 x FBP
Ligand OXM 8 x OXM
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 84.9Å b: 118.2Å c: 135.5Å
α: 90° β: 96.07° γ: 90°
R-values:
R R work R free
0.157 not available not available
Expression system: Not provided

Quick links

Citations

4 review citations

Fold change in evolution of protein structures.
Grishin NV. (2001)
3 more

18 mentions without citation

Molecular dynamics simulations: advances and applications.
Hospital et al. (2015)
17 more