› 1ldo

X-ray diffraction
2.2Å resolution

avidin-norbioitn complex

Released:
DOI: 10.2210/pdb1ldo/pdb
PDB entry 1ldo coloured by chain, front view.
PDB entry 1ldo coloured by chain, side view.
PDB entry 1ldo coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Ligand exchange between proteins. Exchange of biotin and biotin derivatives between avidin and streptavidin.
Pazy Y, Kulik T, Bayer EA, Wilchek M, Livnah O
J Biol Chem 277 30892-900 (2002)
PMID: 12055191

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136083 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Avidin Chains: A, B
Molecule details ›
Chains: A, B
Length: 128 amino acids
Theoretical weight: 14.35 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P02701 (Residues: 25-152; Coverage: 100%)
Gene name: AVD
Sequence domains: Avidin family
Structure domains: Avidin-like

Ligands and Environments

2 bound ligands:
Ligand NAG 2 x NAG
Ligand SNR 2 x SNR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P21212
Unit cell:
a: 71.103Å b: 79.813Å c: 43.14Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.183 0.241

Quick links

Citations

3 review citations

Essentials of biorecognition: the (strept)avidin-biotin system as a model for protein-protein and protein-ligand interaction.
Wilchek et al. (2006)
2 more

1 mention without citation

The mutation T477A in HIV-1 reverse transcriptase (RT) restores normal proteolytic processing of RT in virus with Gag-Pol mutated in the p51-RNH cleavage site.
Abram et al. (2010)