› 1lta

X-ray diffraction
2.2Å resolution

2.2 ANGSTROMS CRYSTAL STRUCTURE OF E. COLI HEAT-LABILE ENTEROTOXIN (LT) WITH BOUND GALACTOSE

Released:
DOI: 10.2210/pdb1lta/pdb
PDB entry 1lta coloured by chain, front view.
PDB entry 1lta coloured by chain, side view.
PDB entry 1lta coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Galactose-binding site in Escherichia coli heat-labile enterotoxin (LT) and cholera toxin (CT).
Merritt EA, Sixma TK, Kalk KH, van Zanten BA, Hol WG
Mol Microbiol 13 745-53 (1994)
PMID: 7997185

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139157 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Heat-labile enterotoxin B chain Chains: D, E, F, G, H
Molecule details ›
Chains: D, E, F, G, H
Length: 103 amino acids
Theoretical weight: 11.81 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P32890 (Residues: 22-124; Coverage: 100%)
Gene names: eltB, ltpB
Sequence domains: Heat-labile enterotoxin beta chain
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)
Heat-labile enterotoxin A chain Chain: A
Molecule details ›
Chain: A
Length: 188 amino acids
Theoretical weight: 21.64 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P06717 (Residues: 19-206; Coverage: 78%)
Gene names: eltA, ltpA
Sequence domains: Heat-labile enterotoxin alpha chain
Structure domains: Heat-Labile Enterotoxin, subunit A
Heat-labile enterotoxin A chain Chain: C
Molecule details ›
Chain: C
Length: 49 amino acids
Theoretical weight: 5.94 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P06717 (Residues: 210-258; Coverage: 20%)
Gene names: eltA, ltpA
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

1 bound ligand:
Ligand GAL 5 x GAL
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 70.7Å b: 73.5Å c: 163.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.175 not available
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Interaction of membrane/lipid rafts with the cytoskeleton: impact on signaling and function: membrane/lipid rafts, mediators of cytoskeletal arrangement and cell signaling.
Head et al. (2014)
16 more

8 mentions without citation

Heat-labile enterotoxin: beyond G(m1) binding.
Mudrak et al. (2010)
7 more