1ltb

X-ray diffraction
2.6Å resolution

2.6 ANGSTROMS CRYSTAL STRUCTURE OF PARTIALLY-ACTIVATED E. COLI HEAT-LABILE ENTEROTOXIN (LT)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
PDBe Complex ID:
PDB-CPX-139157 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Heat-labile enterotoxin B chain Chains: D, E, F, G, H
Molecule details ›
Chains: D, E, F, G, H
Length: 103 amino acids
Theoretical weight: 11.81 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P32890 (Residues: 22-124; Coverage: 100%)
Gene names: eltB, ltpB
Sequence domains: Heat-labile enterotoxin beta chain
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)
Heat-labile enterotoxin A chain Chain: A
Molecule details ›
Chain: A
Length: 185 amino acids
Theoretical weight: 21.35 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P06717 (Residues: 22-206; Coverage: 77%)
Gene names: eltA, ltpA
Sequence domains: Heat-labile enterotoxin alpha chain
Structure domains: Heat-Labile Enterotoxin, subunit A
Heat-labile enterotoxin A chain Chain: C
Molecule details ›
Chain: C
Length: 45 amino acids
Theoretical weight: 5.43 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P06717 (Residues: 210-254; Coverage: 19%)
Gene names: eltA, ltpA
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 119.2Å b: 98.2Å c: 64.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.172 not available
Expression system: Escherichia coli