› 1ltg

X-ray diffraction
2.4Å resolution

THE ARG7LYS MUTANT OF HEAT-LABILE ENTEROTOXIN EXHIBITS GREAT FLEXIBILITY OF ACTIVE SITE LOOP 47-56 OF THE A SUBUNIT

Released:
DOI: 10.2210/pdb1ltg/pdb
PDB entry 1ltg coloured by chain, front view.
PDB entry 1ltg coloured by chain, side view.
PDB entry 1ltg coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The Arg7Lys mutant of heat-labile enterotoxin exhibits great flexibility of active site loop 47-56 of the A subunit.
van den Akker F, Merritt EA, Pizza M, Domenighini M, Rappuoli R, Hol WG
Biochemistry 34 10996-1004 (1995)
PMID: 7669757

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139157 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Heat-labile enterotoxin B chain Chains: D, E, F, G, H
Molecule details ›
Chains: D, E, F, G, H
Length: 103 amino acids
Theoretical weight: 11.81 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P32890 (Residues: 22-124; Coverage: 100%)
Gene names: eltB, ltpB
Sequence domains: Heat-labile enterotoxin beta chain
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)
Heat-labile enterotoxin A chain Chain: A
Molecule details ›
Chain: A
Length: 191 amino acids
Theoretical weight: 21.9 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P06717 (Residues: 19-209; Coverage: 80%)
Gene names: eltA, ltpA
Sequence domains: Heat-labile enterotoxin alpha chain
Structure domains: Heat-Labile Enterotoxin, subunit A
Heat-labile enterotoxin A chain Chain: C
Molecule details ›
Chain: C
Length: 49 amino acids
Theoretical weight: 5.94 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P06717 (Residues: 210-258; Coverage: 20%)
Gene names: eltA, ltpA
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 119.7Å b: 98.5Å c: 65.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.178 not available
Expression system: Escherichia coli

Quick links

Citations

2 review citations

ADP-ribosylation systems in bacteria and viruses.
Mikolčević et al. (2021)
1 more

2 mentions without citation

Estimation of the quality of refined protein crystal structures.
Wang J. (2015)
1 more