› 1m3u

X-ray diffraction
1.8Å resolution

Crystal Structure of Ketopantoate Hydroxymethyltransferase complexed the Product Ketopantoate

Released:
DOI: 10.2210/pdb1m3u/pdb
PDB entry 1m3u coloured by chain, front view.
PDB entry 1m3u coloured by chain, side view.
PDB entry 1m3u coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure of E. coli ketopantoate hydroxymethyl transferase complexed with ketopantoate and Mg2+, solved by locating 160 selenomethionine sites.
von Delft F, Inoue T, Saldanha SA, Ottenhof HH, Schmitzberger F, Birch LM, Dhanaraj V, Witty M, Smith AG, Blundell TL, Abell C
Structure 11 985-96 (2003)
PMID: 12906829

Function and Biology Details

Reaction catalysed: 
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H(2)O = tetrahydrofolate + 2-dehydropantoate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151901 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-methyl-2-oxobutanoate hydroxymethyltransferase Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 264 amino acids
Theoretical weight: 28.26 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P31057 (Residues: 1-264; Coverage: 100%)
Gene names: JW0130, b0134, panB
Sequence domains: Ketopantoate hydroxymethyltransferase
Structure domains: Phosphoenolpyruvate-binding domains

Ligands and Environments

2 bound ligands:
Ligand MG 10 x MG
Ligand KPL 10 x KPL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 86.074Å b: 157.17Å c: 100.181Å
α: 90° β: 97.44° γ: 90°
R-values:
R R work R free
0.158 0.152 0.193
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Coenzyme A biosynthesis: an antimicrobial drug target.
Spry et al. (2008)
3 more

7 mentions without citation

The Blueprint of a Minimal Cell: MiniBacillus.
Reuß et al. (2016)
6 more