› 1mc0

X-ray diffraction
2.86Å resolution

Regulatory Segment of Mouse 3',5'-Cyclic Nucleotide Phosphodiesterase 2A, Containing the GAF A and GAF B Domains

Released:
DOI: 10.2210/pdb1mc0/pdb
PDB entry 1mc0 coloured by chain, front view.
PDB entry 1mc0 coloured by chain, side view.
PDB entry 1mc0 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
The two GAF domains in phosphodiesterase 2A have distinct roles in dimerization and in cGMP binding.
Martinez SE, Wu AY, Glavas NA, Tang XB, Turley S, Hol WG, Beavo JA
Proc Natl Acad Sci U S A 99 13260-5 (2002)
PMID: 12271124

Function and Biology Details

Reaction catalysed: 
Nucleoside 3',5'-cyclic phosphate + H(2)O = nucleoside 5'-phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-187651 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cGMP-dependent 3',5'-cyclic phosphodiesterase Chain: A
Molecule details ›
Chain: A
Length: 368 amino acids
Theoretical weight: 41.42 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q922S4 (Residues: 209-572; Coverage: 38%)
Gene name: Pde2a
Sequence domains:
Structure domains: Beta-Lactamase

Ligands and Environments

1 bound ligand:
Ligand PCG 1 x PCG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: F222
Unit cell:
a: 134.2Å b: 136.2Å c: 149.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.221 0.266
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

49 review citations

Biochemistry and physiology of cyclic nucleotide phosphodiesterases: essential components in cyclic nucleotide signaling.
Conti et al. (2007)
48 more

13 mentions without citation

Cyclic nucleotide binding GAF domains from phosphodiesterases: structural and mechanistic insights.
Heikaus et al. (2009)
12 more