› 1mj0

X-ray diffraction
2.03Å resolution

SANK E3_5: an artificial Ankyrin repeat protein

Released:
DOI: 10.2210/pdb1mj0/pdb
PDB entry 1mj0 coloured by chain, front view.
PDB entry 1mj0 coloured by chain, side view.
PDB entry 1mj0 coloured by chain, top view.
Source organism: Designed synthetic gene
Primary publication:
Designed to be stable: crystal structure of a consensus ankyrin repeat protein.
Kohl A, Binz HK, Forrer P, Stumpp MT, Plückthun A, Grütter MG
Proc Natl Acad Sci U S A 100 1700-5 (2003)
PMID: 12566564

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162328 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SANK E3_5 Protein Chain: A
Molecule details ›
Chain: A
Length: 166 amino acids
Theoretical weight: 17.74 KDa
Source organism: Designed synthetic gene
Expression system: Escherichia coli
Structure domains: Ankyrin repeat-containing domain

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: P21212
Unit cell:
a: 73.864Å b: 47.36Å c: 47.003Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.179 0.23
Expression system: Escherichia coli

Quick links

Citations

39 review citations

The ankyrin repeat as molecular architecture for protein recognition.
Mosavi et al. (2004)
38 more

18 mentions without citation

In vitro-engineered non-antibody protein therapeutics.
Simeon et al. (2018)
17 more