› 1mni

X-ray diffraction
2.07Å resolution

ALTERATION OF AXIAL COORDINATION BY PROTEIN ENGINEERING IN MYOGLOBIN. BIS-IMIDAZOLE LIGATION IN THE HIS64-->VAL(SLASH)VAL68-->HIS DOUBLE MUTANT

Released:
DOI: 10.2210/pdb1mni/pdb
PDB entry 1mni coloured by chain, front view.
PDB entry 1mni coloured by chain, side view.
PDB entry 1mni coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
Alteration of axial coordination by protein engineering in myoglobin. Bisimidazole ligation in the His64-->Val/Val68-->His double mutant.
Dou Y, Admiraal SJ, Ikeda-Saito M, Krzywda S, Wilkinson AJ, Li T, Olson JS, Prince RC, Pickering IJ, George GN
J Biol Chem 270 15993-6001 (1995)
PMID: 7608158

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135413 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Myoglobin Chains: A, B
Molecule details ›
Chains: A, B
Length: 153 amino acids
Theoretical weight: 16.98 KDa
Source organism: Sus scrofa
Expression system: Not provided
UniProt:
  • Canonical: P02189 (Residues: 2-154; Coverage: 99%)
Gene name: MB
Sequence domains: Globin
Structure domains: Globins

Ligands and Environments

1 bound ligand:
Ligand HEM 2 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
Unit cell:
a: 124.49Å b: 42.28Å c: 92.27Å
α: 90° β: 92.62° γ: 90°
R-values:
R R work R free
0.175 not available not available
Expression system: Not provided

Quick links

Citations

4 review citations

Structure and reactivity of hexacoordinate hemoglobins.
Kakar et al. (2010)
3 more

3 mentions without citation

Metalloproteins containing cytochrome, iron-sulfur, or copper redox centers.
Liu et al. (2014)
2 more