› 1mqo

X-ray diffraction
1.35Å resolution

Metallo-beta-lactamase BcII Cd substituted from Bacillus cereus at 1.35 angstroms resolution

Released:
DOI: 10.2210/pdb1mqo/pdb
PDB entry 1mqo coloured by chain, front view.
PDB entry 1mqo coloured by chain, side view.
PDB entry 1mqo coloured by chain, top view.
Source organism: Bacillus cereus
Entry authors: Garcia-Saez I, Chantalat L, Dideberg O

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137499 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Metallo-beta-lactamase type 2 Chain: A
Molecule details ›
Chain: A
Length: 227 amino acids
Theoretical weight: 25 KDa
Source organism: Bacillus cereus
Expression system: Escherichia coli
UniProt:
  • Canonical: P04190 (Residues: 31-257; Coverage: 100%)
Gene name: blm
Sequence domains: Metallo-beta-lactamase superfamily
Structure domains: Ribonuclease Z/Hydroxyacylglutathione hydrolase-like

Ligands and Environments

2 bound ligands:
Ligand CD 3 x CD
Ligand CIT 1 x CIT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: C2
Unit cell:
a: 53.26Å b: 61.38Å c: 69.65Å
α: 90° β: 93.24° γ: 90°
R-values:
R R work R free
0.222 0.222 0.248
Expression system: Escherichia coli

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