› 1mve

X-ray diffraction
1.7Å resolution

Crystal structure of a natural circularly-permutated jellyroll protein: 1,3-1,4-beta-D-glucanase from Fibrobacter succinogenes

Released:
DOI: 10.2210/pdb1mve/pdb
PDB entry 1mve coloured by chain, front view.
PDB entry 1mve coloured by chain, side view.
PDB entry 1mve coloured by chain, top view.
Source organism: Fibrobacter succinogenes
Primary publication:
Crystal structure of a natural circularly permuted jellyroll protein: 1,3-1,4-beta-D-glucanase from Fibrobacter succinogenes.
Tsai LC, Shyur LF, Lee SH, Lin SS, Yuan HS
J Mol Biol 330 607-20 (2003)
PMID: 12842475

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148237 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-glucanase Chain: A
Molecule details ›
Chain: A
Length: 243 amino acids
Theoretical weight: 27.37 KDa
Source organism: Fibrobacter succinogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: P17989 (Residues: 25-266; Coverage: 74%)
Gene names: FSU_0226, Fisuc_2961
Sequence domains: Glycosyl hydrolases family 16
Structure domains: Jelly Rolls

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-18B
Spacegroup: P212121
Unit cell:
a: 40.85Å b: 73.35Å c: 73.71Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.192 0.237
Expression system: Escherichia coli

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Citations

1 review citation

The features that distinguish lichenases from other polysaccharide-hydrolyzing enzymes and the relevance of lichenases for biotechnological applications.
Goldenkova-Pavlova et al. (2018)
29 other articles

1 mention without citation

Functional classification of protein structures by local structure matching in graph representation.
Mills et al. (2018)