› 1mwk

X-ray diffraction
2.3Å resolution

ParM from plasmid R1 APO form

Released:
DOI: 10.2210/pdb1mwk/pdb
PDB entry 1mwk coloured by chain, front view.
PDB entry 1mwk coloured by chain, side view.
PDB entry 1mwk coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
F-actin-like filaments formed by plasmid segregation protein ParM.
van den Ent F, Møller-Jensen J, Amos LA, Gerdes K, Löwe J
EMBO J 21 6935-43 (2002)
PMID: 12486014

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145973 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Plasmid segregation protein ParM Chains: A, B
Molecule details ›
Chains: A, B
Length: 320 amino acids
Theoretical weight: 35.8 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P11904 (Residues: 1-320; Coverage: 100%)
Gene names: parM, stbA
Sequence domains:
Structure domains: Nucleotidyltransferase; domain 5

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21212
Unit cell:
a: 117.68Å b: 72.46Å c: 87.56Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.217 0.266
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

59 review citations

Actin structure and function.
Dominguez et al. (2011)
58 more

12 mentions without citation

Structural frameworks for considering microbial protein- and nucleic acid-dependent motor ATPases.
Thomsen et al. (2008)
11 more