› 1mzj

X-ray diffraction
2.1Å resolution

Crystal Structure of the Priming beta-Ketosynthase from the R1128 Polyketide Biosynthetic Pathway

Released:
DOI: 10.2210/pdb1mzj/pdb
PDB entry 1mzj coloured by chain, front view.
PDB entry 1mzj coloured by chain, side view.
PDB entry 1mzj coloured by chain, top view.
Source organism: Streptomyces sp. R1128
Primary publication:
Crystal structure of the priming beta-ketosynthase from the R1128 polyketide biosynthetic pathway.
Pan H, Tsai Sc, Meadows ES, Miercke LJ, Keatinge-Clay AT, O'Connell J, Khosla C, Stroud RM
Structure 10 1559-68 (2002)
PMID: 12429097

Function and Biology Details

Reactions catalysed: 
Acetyl-CoA + malonyl-[acyl-carrier-protein] = acetoacetyl-[acyl-carrier-protein] + CoA + CO(2) 
(2S)-2-methylbutanoyl-CoA + a malonyl-[acyl-carrier protein] = a (4S)-4-methyl-3-oxohexanoyl-[acyl-carrier-protein] + CoA + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190262 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-ketoacyl-[acyl-carrier-protein] synthase III Chains: A, B
Molecule details ›
Chains: A, B
Length: 339 amino acids
Theoretical weight: 35.43 KDa
Source organism: Streptomyces sp. R1128
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9F6D4 (Residues: 1-339; Coverage: 100%)
Gene names: fabH, zhuH
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments


Cofactor: Ligand COA 2 x COA
1 bound ligand:
Ligand ACE 2 x ACE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P31
Unit cell:
a: 96Å b: 96Å c: 72.6Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.217 0.215 0.251
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

14 review citations

The structural biology of type II fatty acid biosynthesis.
White et al. (2005)
13 more

4 mentions without citation

Biosynthesis of aromatic polyketides in bacteria.
Das et al. (2009)
3 more