› 1n59

X-ray diffraction
2.95Å resolution

Crystal structure of the Murine class I Major Histocompatibility Complex of H-2KB, B2-Microglobulin, and A 9-Residue immunodominant peptide epitope gp33 derived from LCMV

Released:
DOI: 10.2210/pdb1n59/pdb
PDB entry 1n59 coloured by chain, front view.
PDB entry 1n59 coloured by chain, side view.
PDB entry 1n59 coloured by chain, top view.
Source organisms:
Primary publication:
A structural basis for LCMV immune evasion: subversion of H-2D(b) and H-2K(b) presentation of gp33 revealed by comparative crystal structure.Analyses.
Achour A, Michaëlsson J, Harris RA, Odeberg J, Grufman P, Sandberg JK, Levitsky V, Kärre K, Sandalova T, Schneider G
Immunity 17 757-68 (2002)
PMID: 12479822

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-135065 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
H-2 class I histocompatibility antigen, K-B alpha chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 276 amino acids
Theoretical weight: 31.87 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01901 (Residues: 22-297; Coverage: 79%)
Gene names: H2-K, H2-K1
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, D
Molecule details ›
Chains: B, D
Length: 99 amino acids
Theoretical weight: 11.7 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01887 (Residues: 21-119; Coverage: 100%)
Gene name: B2m
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Stable signal peptide Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 9 amino acids
Theoretical weight: 1.05 KDa
Source organism: Mammarenavirus choriomeningitidis
Expression system: Not provided
UniProt:
  • Canonical: Q9QDK7 (Residues: 33-41; Coverage: 2%)
Gene name: GPC

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: P21
Unit cell:
a: 50.551Å b: 88.574Å c: 120.09Å
α: 90° β: 93.72° γ: 90°
R-values:
R R work R free
0.232 0.229 0.287
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Molecular pathways for antigenic peptide generation by ER aminopeptidase 1.
Mpakali et al. (2019)
1 more