› 1n9k

X-ray diffraction
2.2Å resolution

Crystal structure of the bromide adduct of AphA class B acid phosphatase/phosphotransferase from E. coli at 2.2 A resolution

Released:
DOI: 10.2210/pdb1n9k/pdb
PDB entry 1n9k coloured by chain, front view.
PDB entry 1n9k coloured by chain, side view.
PDB entry 1n9k coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The first structure of a bacterial class B Acid phosphatase reveals further structural heterogeneity among phosphatases of the haloacid dehalogenase fold.
Calderone V, Forleo C, Benvenuti M, Cristina Thaller M, Rossolini GM, Mangani S
J Mol Biol 335 761-73 (2004)
PMID: 14687572

Function and Biology Details

Reaction catalysed: 
A phosphate monoester + H(2)O = an alcohol + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142401 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Class B acid phosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 212 amino acids
Theoretical weight: 23.56 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AE22 (Residues: 26-237; Coverage: 100%)
Gene names: JW4015, aphA, b4055, napA, yjbP
Sequence domains: HAD superfamily, subfamily IIIB (Acid phosphatase)
Structure domains: HAD superfamily/HAD-like

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand BR 15 x BR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X31
Spacegroup: P21212
Unit cell:
a: 49.499Å b: 92.616Å c: 138.247Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.189 0.231
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Reactions and structural characterization of gold(III) complexes with amino acids, peptides and proteins.
Glišić et al. (2012)
14 other articles

2 mentions without citation

Mechanisms of protein evolution.
Jayaraman et al. (2022)
1 more