1nff

X-ray diffraction
1.8Å resolution

Crystal structure of Rv2002 gene product from Mycobacterium tuberculosis

Released:

Function and Biology Details

Reaction catalysed:
Androstan-3-alpha,17-beta-diol + NAD(+) = 17-beta-hydroxyandrostan-3-one + NADH
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
PDBe Complex ID:
PDB-CPX-161513 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 260 amino acids
Theoretical weight: 27.1 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WGT1 (Residues: 1-260; Coverage: 100%)
Gene names: MTCY39.16c, Rv2002, fabG3
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-18B
Spacegroup: P3221
Unit cell:
a: 70.377Å b: 70.377Å c: 148.934Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.164 0.164 0.199
Expression system: Escherichia coli