› 1nfk

X-ray diffraction
2.3Å resolution

STRUCTURE OF THE NUCLEAR FACTOR KAPPA-B (NF-KB) P50 HOMODIMER

Released:
DOI: 10.2210/pdb1nfk/pdb
PDB entry 1nfk coloured by chain, front view.
PDB entry 1nfk coloured by chain, side view.
PDB entry 1nfk coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structure of NF-kappa B p50 homodimer bound to a kappa B site.
Ghosh G, van Duyne G, Ghosh S, Sigler PB
Nature 373 303-10 (1995)
PMID: 7530332

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114243 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Nuclear factor NF-kappa-B p50 subunit Chains: A, B
Molecule details ›
Chains: A, B
Length: 325 amino acids
Theoretical weight: 36.6 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P25799 (Residues: 39-363; Coverage: 34%)
Gene name: Nfkb1
Sequence domains:
Structure domains:
DNA (5'-D(*TP*GP*GP*GP*AP*AP*TP*TP*CP*CP*C)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 11 nucleotides
Theoretical weight: 3.35 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: C2
Unit cell:
a: 84.2Å b: 132.1Å c: 80.1Å
α: 90° β: 93.1° γ: 90°
R-values:
R R work R free
0.23 0.23 0.34
Expression system: Not provided

Quick links

Citations

84 review citations

The NF-kappa B and I kappa B proteins: new discoveries and insights.
Baldwin AS. (1996)
83 more

61 mentions without citation

Nucleic acid nanoparticles (NANPs) as molecular tools to direct desirable and avoid undesirable immunological effects.
Johnson et al. (2021)
60 more