› 1ng1

X-ray diffraction
2.03Å resolution

N AND GTPASE DOMAINS OF THE SIGNAL SEQUENCE RECOGNITION PROTEIN FFH FROM THERMUS AQUATICUS

Released:
DOI: 10.2210/pdb1ng1/pdb
PDB entry 1ng1 coloured by chain, front view.
PDB entry 1ng1 coloured by chain, side view.
PDB entry 1ng1 coloured by chain, top view.
Source organism: Thermus aquaticus
Primary publication:
Functional changes in the structure of the SRP GTPase on binding GDP and Mg2+GDP.
Freymann DM, Keenan RJ, Stroud RM, Walter P
Nat Struct Biol 6 793-801 (1999)
PMID: 10426959

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126575 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Signal recognition particle protein Chain: A
Molecule details ›
Chain: A
Length: 294 amino acids
Theoretical weight: 32.3 KDa
Source organism: Thermus aquaticus
Expression system: Escherichia coli
UniProt:
  • Canonical: O07347 (Residues: 1-294; Coverage: 68%)
Gene name: ffh
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand CD 3 x CD
Ligand MG 2 x MG
Ligand GDP 1 x GDP
Ligand EDO 3 x EDO
Ligand ACY 4 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P43212
Unit cell:
a: 100.072Å b: 100.072Å c: 73.49Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.189 0.25
Expression system: Escherichia coli

Quick links

Citations

14 review citations

The signal recognition particle.
Keenan et al. (2001)
13 more

12 mentions without citation

Structure, function and evolution of the signal recognition particle.
Nagai et al. (2003)
11 more