› 1nhu

X-ray diffraction
2Å resolution

Hepatitis C virus RNA polymerase in complex with non-nucleoside analogue inhibitor

Released:
DOI: 10.2210/pdb1nhu/pdb
PDB entry 1nhu coloured by chain, front view.
PDB entry 1nhu coloured by chain, side view.
PDB entry 1nhu coloured by chain, top view.
Source organism: Hepatitis C virus subtype 1b
Primary publication:
Non-nucleoside analogue inhibitors bind to an allosteric site on HCV NS5B polymerase. Crystal structures and mechanism of inhibition.
Wang M, Ng KK, Cherney MM, Chan L, Yannopoulos CG, Bedard J, Morin N, Nguyen-Ba N, Alaoui-Ismaili MH, Bethell RC, James MN
J Biol Chem 278 9489-95 (2003)
PMID: 12509436

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Hydrolysis of four peptide bonds in the viral precursor polyprotein, commonly with Asp or Glu in the P6 position, Cys or Thr in P1 and Ser or Ala in P1'.
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150808 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-directed RNA polymerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 578 amino acids
Theoretical weight: 64.26 KDa
Source organism: Hepatitis C virus subtype 1b
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P26663 (Residues: 2420-2989; Coverage: 19%)
Sequence domains: Viral RNA dependent RNA polymerase
Structure domains: Alpha-Beta Plaits

Ligands and Environments

1 bound ligand:
Ligand 153 2 x 153
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P212121
Unit cell:
a: 86.015Å b: 104.684Å c: 126.843Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.219 0.256
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

18 review citations

Challenges and successes in developing new therapies for hepatitis C.
De Francesco et al. (2005)
17 more

9 mentions without citation

RNA Dependent RNA Polymerases: Insights from Structure, Function and Evolution.
Venkataraman et al. (2018)
8 more