› 1njg

X-ray diffraction
2.2Å resolution

Nucleotide-free form of an Isolated E. coli Clamp Loader Gamma Subunit

Released:
DOI: 10.2210/pdb1njg/pdb
PDB entry 1njg coloured by chain, front view.
PDB entry 1njg coloured by chain, side view.
PDB entry 1njg coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Nucleotide-induced conformational changes in an isolated Escherichia coli DNA polymerase III clamp loader subunit.
Podobnik M, Weitze TF, O'Donnell M, Kuriyan J
Structure 11 253-63 (2003)
PMID: 12623013

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139152 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA polymerase III subunit tau Chains: A, B
Molecule details ›
Chains: A, B
Length: 250 amino acids
Theoretical weight: 27.5 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06710 (Residues: 1-243; Coverage: 38%)
Gene names: JW0459, b0470, dnaX, dnaZ, dnaZX
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21
Unit cell:
a: 47.335Å b: 85.471Å c: 56.932Å
α: 90° β: 93.15° γ: 90°
R-values:
R R work R free
0.238 0.238 0.268
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Cellular DNA replicases: components and dynamics at the replication fork.
Johnson et al. (2005)
8 more

7 mentions without citation

The Blueprint of a Minimal Cell: MiniBacillus.
Reuß et al. (2016)
6 more