› 1npj

X-ray diffraction
1.9Å resolution

Crystal structure of H145A mutant of nitrite reductase from Alcaligenes faecalis

Released:
DOI: 10.2210/pdb1npj/pdb
PDB entry 1npj coloured by chain, front view.
PDB entry 1npj coloured by chain, side view.
PDB entry 1npj coloured by chain, top view.
Source organism: Alcaligenes faecalis
Primary publication:
Reconstitution of the type-1 active site of the H145G/A variants of nitrite reductase by ligand insertion.
Wijma HJ, Boulanger MJ, Molon A, Fittipaldi M, Huber M, Murphy ME, Verbeet MP, Canters GW
Biochemistry 42 4075-83 (2003)
PMID: 12680761

Function and Biology Details

Reaction catalysed: 
Nitric oxide + H(2)O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153659 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Copper-containing nitrite reductase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 343 amino acids
Theoretical weight: 37 KDa
Source organism: Alcaligenes faecalis
Expression system: Escherichia coli
UniProt:
  • Canonical: P38501 (Residues: 34-376; Coverage: 100%)
Gene names: nir, nirK
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

1 bound ligand:
Ligand CU 6 x CU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 61.6Å b: 102.52Å c: 146.07Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.177 0.225
Expression system: Escherichia coli

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Citations

1 review citation

Copper active sites in biology.
Solomon et al. (2014)
11 other articles