› 1nsm

X-ray diffraction
1.85Å resolution

Crystal structure of galactose mutarotase from Lactococcus lactis mutant D243A complexed with galactose

Released:
DOI: 10.2210/pdb1nsm/pdb
PDB entry 1nsm coloured by chain, front view.
PDB entry 1nsm coloured by chain, side view.
PDB entry 1nsm coloured by chain, top view.
Source organism: Lactococcus lactis
Primary publication:
The catalytic mechanism of galactose mutarotase.
Thoden JB, Kim J, Raushel FM, Holden HM
Protein Sci 12 1051-9 (2003)
PMID: 12717027

Function and Biology Details

Reaction catalysed: 
Alpha-D-glucose = beta-D-glucose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195605 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aldose 1-epimerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 347 amino acids
Theoretical weight: 38.58 KDa
Source organism: Lactococcus lactis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9ZB17 (Residues: 1-339; Coverage: 100%)
Gene name: galM
Sequence domains: Aldose 1-epimerase
Structure domains: Beta-galactosidase; Chain A, domain 5

Ligands and Environments

3 bound ligands:
Ligand GAL 1 x GAL
Ligand GLA 1 x GLA
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 44.8Å b: 76.3Å c: 211Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.176 0.236
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Structure and function of enzymes of the Leloir pathway for galactose metabolism.
Holden et al. (2003)
1 more

3 mentions without citation

The catalytic mechanism of galactose mutarotase.
Thoden et al. (2003)
2 more