› 1o08

X-ray diffraction
1.2Å resolution

Structure of Pentavalent Phosphorous Intermediate of an Enzyme Catalyzed Phosphoryl transfer Reaction observed on cocrystallization with Glucose 1-phosphate

Released:
DOI: 10.2210/pdb1o08/pdb
PDB entry 1o08 coloured by chain, front view.
PDB entry 1o08 coloured by chain, side view.
PDB entry 1o08 coloured by chain, top view.
Source organism: Lactococcus lactis
Primary publication:
The pentacovalent phosphorus intermediate of a phosphoryl transfer reaction.
Lahiri SD, Zhang G, Dunaway-Mariano D, Allen KN
Science 299 2067-71 (2003)
PMID: 12637673

Function and Biology Details

Reaction catalysed: 
Beta-D-glucose 1-phosphate = beta-D-glucose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159791 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-phosphoglucomutase Chain: A
Molecule details ›
Chain: A
Length: 221 amino acids
Theoretical weight: 24.24 KDa
Source organism: Lactococcus lactis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P71447 (Residues: 1-221; Coverage: 100%)
Gene names: L0001, LL0429, pgmB
Sequence domains: haloacid dehalogenase-like hydrolase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand G16 1 x G16
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P212121
Unit cell:
a: 36.939Å b: 54.297Å c: 104.68Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.141 0.141 0.179
Expression system: Escherichia coli BL21

Quick links

Citations

25 review citations

Serine/threonine phosphatases: mechanism through structure.
Shi Y. (2009)
24 more

18 mentions without citation

Determinants for dephosphorylation of the RNA polymerase II C-terminal domain by Scp1.
Zhang et al. (2006)
17 more