› 1o7j

X-ray diffraction
1Å resolution

Atomic resolution structure of Erwinia chrysanthemi L-asparaginase

Released:
DOI: 10.2210/pdb1o7j/pdb
PDB entry 1o7j coloured by chain, front view.
PDB entry 1o7j coloured by chain, side view.
PDB entry 1o7j coloured by chain, top view.
Source organism: Dickeya chrysanthemi
Primary publication:
Atomic resolution structure of Erwinia chrysanthemi L-asparaginase.
Lubkowski J, Dauter M, Aghaiypour K, Wlodawer A, Dauter Z
Acta Crystallogr D Biol Crystallogr 59 84-92 (2003)
PMID: 12499544

Function and Biology Details

Reaction catalysed: 
L-asparagine + H(2)O = L-aspartate + NH(3)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139091 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-asparaginase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 327 amino acids
Theoretical weight: 35.12 KDa
Source organism: Dickeya chrysanthemi
UniProt:
  • Canonical: P06608 (Residues: 22-348; Coverage: 100%)
Gene names: ansB, asn
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SO4 8 x SO4
Ligand GOL 2 x GOL
Ligand EDO 12 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: C2
Unit cell:
a: 106.38Å b: 90.35Å c: 127.59Å
α: 90° β: 91.4° γ: 90°
R-values:
R R work R free
0.11 not available 0.128

Quick links

Citations

8 review citations

A Comprehensive Review on L-Asparaginase and Its Applications.
Batool et al. (2016)
7 more

9 mentions without citation

Protein crystallography for non-crystallographers, or how to get the best (but not more) from published macromolecular structures.
Wlodawer et al. (2008)
8 more